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AAT Bioquest

FAQ

Posted on March 18, 2024
What is fluorescence resonance energy transfer used for?
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesPhysiological Probes


Posted on March 18, 2024
What methods are used to measure FRET for cell biology studies?
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Physiological Probes


Posted on March 18, 2024
What factors can influence FRET efficiency?
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Physiological Probes


Posted on March 18, 2024
What should I consider when selecting donor/acceptor FRET pairs?
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Physiological Probes


Posted on March 18, 2024
What are the properties of probes that determine their suitability for FRET applications?
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Physiological Probes


Posted on March 18, 2024
What are the principles of FRET?
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Physiological Probes


Posted on August 29, 2023
How does a molecular beacon work?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What is the importance of molecular beacons?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the advantages of molecular beacon probes?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the limitations of molecular beacon probes?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
Why are MBs particularly suited for mRNA studies?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the strategies for minimizing MB false-positive signals in living cells?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the properties of molecular beacons that affect nucleic acid detection?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the widely used detection methods in quantitative PCR (Q-PCR)
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the best ways to store a molecular beacon preparation?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on August 7, 2023
What guidelines should I follow for successful tandem dye usage?
Chemical Reagents Förster Resonance Energy Transfer (FRET)Antibody and Protein LabelingFlow Cytometry ReagentsPE and APC


Posted on July 6, 2023
Why do some molecules have more energy than others?
Förster Resonance Energy Transfer (FRET)Energy


Posted on May 24, 2023
What are the common methods available to detect kinase activities?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA) Förster Resonance Energy Transfer (FRET)EnzymesFluorescenceProteinReal-Time PCR (qPCR)qPCR


Posted on January 13, 2023
What are the differences between static quenching and dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesPhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What are the applications of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesDNADNA and RNA QuantitationFluorescencePhysiological Probes


Posted on January 13, 2023
What is the mechanism of dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What are the types of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 12, 2023
What are the factors that cause fluorescence quenching?
Bioconjugation Förster Resonance Energy Transfer (FRET)Chemical ReagentsFluorescencePhysiological ProbesTide Quencher Dyes


Posted on January 6, 2023
Which MMPs are collagenases?
Antibodies and Proteomics Bioconjugation Förster Resonance Energy Transfer (FRET)Antibody and Protein LabelingEnzymesMMPProtein


Posted on January 6, 2023
What are the MMPs enzymes in cells?
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Antibody and Protein LabelingEnzymesMMPProtein


Posted on January 5, 2023
What are the main types of cell surface receptors?
Förster Resonance Energy Transfer (FRET)Cell SignalingCellular ProcessesEnzymes


Posted on January 5, 2023
How are soluble MMPs produced?
Förster Resonance Energy Transfer (FRET)Cell SignalingCellular ProcessesEnzymes


Posted on January 5, 2023
What is the basic molecular structure of MMPs?
Förster Resonance Energy Transfer (FRET)Cell SignalingCellular ProcessesEnzymes


Posted on January 5, 2023
How many types of MMPs are there?
Förster Resonance Energy Transfer (FRET)Cell SignalingCellular ProcessesEnzymes


Posted on January 5, 2023
How do TIMPs inhibit MMPs?
Förster Resonance Energy Transfer (FRET)Cell SignalingCellular ProcessesEnzymes


Posted on January 5, 2023
What are the MMPs inhibitors?
Förster Resonance Energy Transfer (FRET)Cell SignalingCellular ProcessesEnzymes


Posted on January 5, 2023
What are the major functions of MMPs?
Förster Resonance Energy Transfer (FRET)Cell SignalingCellular ProcessesEnzymes


Posted on November 14, 2022
What are the similarities between Fluorophore and Chromophore?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)DNA and RNA QuantitationDyes by Functional GroupPCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on June 2, 2022
What is the difference between Tide Quencher 2 (TQ2) and Tide Quencher 2WS (TQ2WS) products?
Tide Quencher Dyes Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingAntibody and Protein Labeling Chemical Reagents


Posted on June 1, 2020
How can I set up compensation for tandem dyes in flow cytometry?
Förster Resonance Energy Transfer (FRET)FRETFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What are tandem dyes in flow cytometry?
Bioconjugation Förster Resonance Energy Transfer (FRET)CyaninesFRETFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry


Posted on May 9, 2020
What are oligonucleotides?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNAFRETPeptide and Oligonucleotidefluorescence imaging